1. 提供詳細且準確的DNA序列
2. 選擇5'/3' 限制酶切位
3. 確認是否要做密碼子優化 (需確認應用物種)
4. 選擇欲放入的載體
Cloning vector
Mammalian expression vector
E coli expression vector
Yeast expression vector
Baculovirus/Insect
Plant expression vector
Gateway vector